# Coral Surface Area and Zooxanthellae Counts # PI: Cara L. Fiore # Version date (v1): 2022-09-21 ========================= Sample_ID Coral_Species TimePoint Treatment Field_Sampling_Date Latitude Longitude Skeletal_fragment_diameter Skeletal_fragment_length ImageJ_side_1 ImageJ_side_2 ImageJ_side_3 ImageJ_side_4 ImageJ_total_surface_area Mass_of_tin_foil Foil_Fragment_Surface_Area Zooxanthellae_Cell_Count Quadrants_counted Total_Volume_of_host_homogenate Volume_of_fixed_zooxanthellae_homogenate_in_Dilution Volume_of_Trypan_Added_to_Dilution Volume_Of_MilliQ_Water_added_to_Dilution Total_volume_added_to_dilution Dilution_Factor Zoox_cells_per_mL_dilution Zoox_cells_per_fragment Surface_area Cells_per_cm2 Notes ------------------------- Ef-Iso-I-1 Eunicea flexuosa T0 Control 2020-12-09 24.558694 -81.503528 0.35 2.2 0.758 0.705 0.673 0.679 2.815 0.0181 2.928553428 208 4 10 0.02 0.002 0 0.002 0.1 52000 520000 2.82 184724.7 2uL trypan blue 20uL sample; 1:2 ratio Ef-iso-I-2 Eunicea flexuosa T0 Control 2020-12-09 24.558694 -81.503528 0.4 2.2 0.704 0.767 0.762 0.756 2.989 0.017 2.750575043 568 4 10 0.02 0.002 0 0.002 0.1 142000 1420000 2.99 475075.3 2uL trypan blue 20uL sample; 1:2 ratio. Picture taken of sample. Ef-iso-I-3 Eunicea flexuosa T0 Control 2020-12-09 24.558694 -81.503528 0.45 2.1 0.868 0.733 0.798 0.739 3.138 0.0182 2.944733281 328 4 10 0.02 0.002 0 0.002 0.1 82000 820000 3.14 261312.9 2uL trypan blue 20uL sample; 1:2 ratio Ef-iso-I-4 Eunicea flexuosa T0 Control 2020-12-09 24.558694 -81.503528 0.4 2.25 0.733 0.708 0.704 0.643 2.788 0.0171 2.766754896 824 4 10 0.02 0.002 0 0.002 0.1 206000 2060000 2.79 738880.9 2uL trypan blue 20uL sample; 1:2 ratio Ef-iso-I-5 Eunicea flexuosa T0 Control 2020-12-09 24.558694 -81.503528 0.6 2.4 0.901 1.004 1.018 1.047 3.97 0.024 3.883164766 825 4 10 0.02 0.002 0 0.002 0.1 206250 2062500 3.97 519521.4 2uL trypan blue 20uL sample; 1:2 ratio. Picture taken of sample. Ef-iso-T3-1 Eunicea flexuosa T3 Control 2020-12-09 24.558694 -81.503528 0.5 2.35 0.866 0.704 0.782 0.66 3.012 0.02 3.235970639 547 4 10 0.02 0.002 0 0.002 0.1 136750 1367500 3.01 454017.3 2uL trypan blue 20uL sample; 1:2 ratio Ef-iso-T3-2 Eunicea flexuosa T3 Enriched 2020-12-09 24.558694 -81.503528 0.4 2.3 0.626 0.79 0.636 0.595 2.647 0.0234 3.786085647 39 4 10 0.02 0.002 0 0.002 0.1 9750 97500 2.65 36834.2 Mostly small skeletal fragments in sample. 2uL trypan blue 20uL sample; 1:2 ratio Ef-iso-T3-3 Eunicea flexuosa T3 Enriched 2020-12-09 24.558694 -81.503528 0.5 2 0.859 0.918 0.849 0.88 3.506 0.024 3.883164766 279 4 10 0.02 0.002 0 0.002 0.1 69750 697500 3.51 198944.7 2uL trypan blue 20uL sample; 1:2 ratio Ef-iso-T3-4 Eunicea flexuosa T3 Control 2020-12-09 24.558694 -81.503528 0.4 2 0.626 0.635 0.667 0.678 2.606 0.0178 2.880013868 361 4 10 0.02 0.002 0 0.002 0.1 90250 902500 2.61 346316.2 2uL trypan blue 20uL sample; 1:2 ratio. Picture taken of sample. Ef-iso-T3-5 Eunicea flexuosa T3 Enriched 2020-12-09 24.558694 -81.503528 0.4 1.98 0.461 0.587 0.597 0.606 2.251 0.018 2.912373575 117 4 10 0.02 0.002 0 0.002 0.1 29250 292500 2.25 129942.2 2uL trypan blue 20uL sample; 1:2 ratio Ef-iso-T3-6 Eunicea flexuosa T3 Enriched 2020-12-09 24.558694 -81.503528 0.4 2.05 0.624 0.521 0.602 0.635 2.382 0.0178 2.880013868 267 4 10 0.02 0.002 0 0.002 0.1 66750 667500 2.38 280226.7 2uL trypan blue 20uL sample; 1:2 ratio Ef-iso-T3-7 Eunicea flexuosa T3 Control 2020-12-09 24.558694 -81.503528 0.5 2.4 0.847 0.841 0.858 0.917 3.463 0.0239 3.866984913 nd nd 10 nd nd nd nd nd nd nd 3.46 nd Side of sample bottle states ""No zoox to be seen on scope"" Ef-iso-T3-8 Eunicea flexuosa T3 Enriched 2020-12-09 24.558694 -81.503528 0.5 2.1 0.89 0.866 0.701 0.671 3.128 0.0214 3.462488583 104 4 10 0.02 0.002 0 0.002 0.1 26000 260000 3.13 83120.2 2uL trypan blue 20uL sample; 1:2 ratio. Picture taken of sample. Ef-iso-T6-1 Eunicea flexuosa T6 Control 2020-12-09 24.558694 -81.503528 0.5 2.2 0.856 0.787 0.873 0.575 3.091 0.0199 3.219790785 671 4 10 0.02 0.002 0 0.002 0.1 167750 1677500 3.09 542704.6 2uL trypan blue 20uL sample; 1:2 ratio. Picture taken of sample. Ef-iso-T6-2 Eunicea flexuosa T6 Enriched 2020-12-09 24.558694 -81.503528 0.4 2.3 0.793 0.764 0.783 0.714 3.054 0.0209 3.381589317 nd nd 10 nd nd nd nd nd nd nd 3.05 nd No zoox cells found in sample ""Eto"" on side of bottle Ef-iso-T6-3 Eunicea flexuosa T6 Enriched 2020-12-09 24.558694 -81.503528 0.5 2 0.764 0.707 0.741 0.784 2.996 0.021 3.39776917 566 4 10 0.02 0.002 0 0.002 0.1 141500 1415000 3 472296.4 2uL trypan blue 20uL sample; 1:2 ratio. Picture taken of sample. Ef-iso-T6-4 Eunicea flexuosa T6 Control 2020-12-09 24.558694 -81.503528 0.4 2.1 0.602 0.633 0.656 0.661 2.552 0.018 2.912373575 573 4 10 0.02 0.002 0 0.002 0.1 143250 1432500 2.55 561324.5 2uL trypan blue 20uL sample; 1:2 ratio Ef-iso-T6-5 Eunicea flexuosa T6 Enriched 2020-12-09 24.558694 -81.503528 0.5 2.1 0.841 0.974 0.882 0.848 3.545 0.0218 3.527207996 628 4 10 0.02 0.002 0 0.002 0.1 157000 1570000 3.55 442877.3 2uL trypan blue 20uL sample; 1:2 ratio Ef-iso-T6-6 Eunicea flexuosa T6 Enriched 2020-12-09 24.558694 -81.503528 0.4 2.2 0.7 0.812 0.867 0.755 3.134 0.0202 3.268330345 1144 4 10 0.02 0.002 0 0.002 0.1 286000 2860000 3.13 912571.8 2uL trypan blue 20uL sample; 1:2 ratio Ef-iso-T6-7 Eunicea flexuosa T6 Control 2020-12-09 24.558694 -81.503528 0.5 2 0.693 0.717 0.692 0.76 2.862 0.0186 3.009452694 945 4 1 0.02 0.002 0 0.002 0.1 236250 236250 2.86 82547.2 2uL trypan blue 20uL sample; 1:2 ratio Ef-iso-T6-8 Eunicea flexuosa T6 Enriched 2020-12-09 24.558694 -81.503528 0.4 2.05 0.766 0.697 0.849 0.904 3.216 0.0207 3.349229611 658 4 10 0.02 0.002 0 0.002 0.1 164500 1645000 3.22 511505 2uL trypan blue 20uL sample; 1:2 ratio Ac-iso-I-1a Acropora cervicornis T0 Control 2020-12-09 24.562747 -81.400455 1.4 9.3 9.162 9.28 8.691 7.926 35.059 0.2742 44.36515745 nd nd nd nd nd nd nd nd nd nd 35.06 nd Did not do zoox counts Ac-iso-I-1b Acropora cervicornis T0 Control 2020-12-09 24.562747 -81.400455 1.4 9 10.175 9.043 8.761 8.621 36.6 0.2495 40.36873372 nd nd nd nd nd nd nd nd nd nd 36.6 nd Dilution Factor 20:100 (1:5) too little cells to count with 1:10 dilution factor. 20uL of sample; 5 uL trypan blue; 75uL MQ water= 100uL Ac-iso-I-1combined for zoox Acropora cervicornis T0 Control 2020-12-09 24.562747 -81.400455 1.4 nd nd nd nd nd 71.659 0.5237 84.73389117 481 4 50 0.02 0.005 0.175 0.18 9 10822500 541125000 71.66 7551389.2 2 fragments were airbrushed for Ac-iso-I-1 and combined so total zoox count must be done using info for both frags Ac-iso-I-2 Acropora cervicornis T0 Control 2020-12-09 24.562747 -81.400455 1.2 8.5 7.636 6.877 7.619 6.876 29.008 0.2318 37.5048997 nd nd 35 nd nd nd nd nd nd nd 29.01 nd Sample was mixed multiple times; unable to achive homogenous solution; needs to be counted Ac-iso-I-3 Acropora cervicornis T0 Control 2020-12-09 24.562747 -81.400455 1.2 9.3 8.378 9.328 9.723 8.438 35.867 0.2532 40.96738828 708 4 45 0.02 0.005 0.175 0.18 9 15930000 716850000 35.87 19986338.4 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Ac-iso-I-4 Acropora cervicornis T0 Control 2020-12-09 24.562747 -81.400455 1.5 10.2 8.564 9.53 10.391 9.325 37.81 0.2707 43.79886259 339 4 28 0.02 0.005 0.175 0.18 9 7627500 213570000 37.81 5648505.7 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Picture taken of sample. Ac-iso-I-5 Acropora cervicornis T0 Control 2020-12-09 24.562747 -81.400455 1.1 8.4 5.939 8.001 7.756 7.042 28.738 0.225 36.40466968 1083 4 40 0.02 0.005 0.175 0.18 9 24367500 974700000 28.74 33916765.3 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Ac-iso-t3-1 Acropora cervicornis T3 Control 2020-12-09 24.562747 -81.400455 1.1 8.3 7.792 7.671 7.9 7.397 30.76 0.2146 34.72196495 902 4 25 0.02 0.005 0.175 0.18 9 20295000 507375000 30.76 16494635.9 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Ac-iso-t3-2 Acropora cervicornis T3 Enriched 2020-12-09 24.562747 -81.400455 1.3 7.9 7.997 7.392 7.799 6.983 30.171 0.2104 34.04241112 950 4 25 0.02 0.005 0.175 0.18 9 21375000 534375000 30.17 17711544.2 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Ac-iso-t3-3 Acropora cervicornis T3 Enriched 2020-12-09 24.562747 -81.400455 1.2 8.8 10.087 8.847 8.633 8.608 36.175 0.2564 41.48514359 943 4 35 0.02 0.005 0.175 0.18 9 21217500 742612500 36.18 20528334.5 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Ac-iso-t3-4 Acropora cervicornis T3 Control 2020-12-09 24.562747 -81.400455 1.2 8.3 7.28 7.656 7.165 7.34 29.441 0.2282 36.92242499 728 4 20 0.02 0.005 0.175 0.18 9 16380000 327600000 29.44 11127339.4 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Ac-iso-t3-5 Acropora cervicornis T3 Enriched 2020-12-09 24.562747 -81.400455 1.4 9.3 8.653 9.985 10.125 9.387 38.15 0.3078 49.80158813 1149 4 30 0.02 0.005 0.175 0.18 9 25852500 775575000 38.15 20329619.9 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Ac-iso-t3-6 Acropora cervicornis T3 Enriched 2020-12-09 24.562747 -81.400455 1.6 9.2 8.093 10.245 11.325 11.434 41.097 0.3168 51.25777491 871 4 25 0.02 0.005 0.175 0.18 9 19597500 489937500 41.1 11921490.6 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Ac-iso-t3-7 Acropora cervicornis T3 Control 2020-12-09 24.562747 -81.400455 1.5 6.8 5.855 7.8 6.789 7.322 27.766 0.2415 39.07434546 553 4 25 0.02 0.005 0.175 0.18 9 12442500 311062500 27.77 11203000.1 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Ac-iso-t3-8 Acropora cervicornis T3 Enriched 2020-12-09 24.562747 -81.400455 1.2 7.8 7.76 7.743 8.074 8.806 32.383 0.241 38.99344619 729 4 28 0.02 0.005 0.175 0.18 9 16402500 459270000 32.38 14182441.4 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Ac-iso-t6-1 Acropora cervicornis T6 Control 2020-12-09 24.562747 -81.400455 1 9.7 8.611 9.164 9.1 9.592 36.467 0.2703 43.73414318 1362 4 25 0.02 0.005 0.175 0.18 9 30645000 766125000 36.47 21008720.2 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Ac-iso-t6-2 Acropora cervicornis T6 Enriched 2020-12-09 24.562747 -81.400455 1.1 8.5 8.282 7.245 7.853 9.322 32.702 0.226 36.56646822 1173 4 38 0.02 0.005 0.175 0.18 9 26392500 1002915000 32.7 30668307.7 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Ac-iso-t6-3 Acropora cervicornis T6 Enriched 2020-12-09 24.562747 -81.400455 1.3 9.6 9.138 9.676 9.128 8.567 36.509 0.2721 44.02538054 791 4 25 0.02 0.005 0.175 0.18 9 17797500 444937500 36.51 12187063.5 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Ac-iso-t6-4 Acropora cervicornis T6 Control 2020-12-09 24.562747 -81.400455 1.3 8.4 7.96 8.599 9.491 8.575 34.625 0.2679 43.3458267 674 4 25 0.02 0.005 0.175 0.18 9 15165000 379125000 34.63 10949458.5 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Ac-iso-t6-5 Acropora cervicornis T6 Enriched 2020-12-09 24.562747 -81.400455 1.3 8.8 8.239 9.069 9.175 8.699 35.182 0.2656 42.97369008 661 4 25 0.02 0.005 0.175 0.18 9 14872500 371812500 35.18 10568259.3 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Ac-iso-t6-6 Acropora cervicornis T6 Enriched 2020-12-09 24.562747 -81.400455 1.2 9.4 8.513 8.526 10.354 9.442 36.835 0.258 41.74402124 630 4 25 0.02 0.005 0.175 0.18 9 14175000 354375000 36.84 9620605.4 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Ac-iso-t6-7 Acropora cervicornis T6 Control 2020-12-09 24.562747 -81.400455 1.3 8.8 8.914 8.475 8.872 8.929 35.19 0.2985 48.29686178 826 4 33 0.02 0.005 0.175 0.18 9 18585000 613305000 35.19 17428388.7 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Picture Taken of sample. Ac-iso-t6-8 Acropora cervicornis T6 Enriched 2020-12-09 24.562747 -81.400455 0.9 9.1 8.341 8.193 8.707 8.001 33.242 0.2776 44.91527246 816 4 35 0.02 0.005 0.175 0.18 9 18360000 642600000 33.24 19330966.8 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Of-iso-I-1 Orbicella faveolata T0 Control nd nd nd 2.9 nd 5.824 nd nd nd 5.824 0.0517 8.364984101 561 4 30 0.02 0.005 0.175 0.18 9 12622500 378675000 5.82 65019745.9 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Of-iso-I-2 Orbicella faveolata T0 Control nd nd nd 2.3 - 2.8 nd 5.922 nd nd nd 5.922 0.0459 7.426552615 295 4 18 0.02 0.005 0.175 0.18 9 6637500 119475000 5.92 20174772 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Of-iso-I-3 Orbicella faveolata T0 Control nd nd nd 2.6 - 3.1 nd 7.33 nd nd nd 7.33 0.0636 10.29038663 325 4 15 0.02 0.005 0.175 0.18 9 7312500 109687500 7.33 14964188.3 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Of-iso-I-4 Orbicella faveolata T0 Control nd nd nd 2.75 - 2.8 nd 5.968 nd nd nd 5.968 0.0475 7.685430266 309 4 22 0.02 0.005 0.175 0.18 9 6952500 152955000 5.97 25629189 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Of-iso-t3-1 Orbicella faveolata T3 Control nd nd nd 3.0 - 3.1 nd 6.809 nd nd nd 6.809 0.0688 11.131739 350 4 20 0.02 0.005 0.175 0.18 9 7875000 157500000 6.81 23131149.9 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Of-iso-t3-2 Orbicella faveolata T3 Enriched nd nd nd 4.0 - 4.5 nd 6.588 nd nd nd 6.588 0.0965 15.61355833 713 4 25 0.02 0.005 0.175 0.18 9 16042500 401062500 6.59 60877732.2 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Picture Taken of sample. Of-iso-t3-3 Orbicella faveolata T3 Control nd nd nd 3.0 - 3.2 nd 8.123 nd nd nd 8.123 0.0592 9.57847309 378 4 12 0.02 0.005 0.175 0.18 9 8505000 102060000 8.12 12564323.5 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Of-iso-t3-4 Orbicella faveolata T3 Enriched nd nd nd 2.5 - 3.0 nd 5.765 nd nd nd 5.765 0.0532 8.607681898 359 4 15 0.02 0.005 0.175 0.18 9 8077500 121162500 5.77 21016912.4 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Of-iso-t3-5 Orbicella faveolata T3 Enriched nd nd nd 3.3 - 3.5 nd 7.141 nd nd nd 7.141 0.0692 11.19645841 295 4 18 0.02 0.005 0.175 0.18 9 6637500 119475000 7.14 16730850 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Of-iso-t3-6 Orbicella faveolata T3 Enriched nd nd nd 3.0 - 3.1 nd 8.067 nd nd nd 8.067 0.0614 9.93442986 352 4 15 0.02 0.005 0.175 0.18 9 7920000 118800000 8.07 14726664.2 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Of-iso-t3-7 Orbicella faveolata T3 Control nd nd nd 3.1 - 3.2 nd 8.932 nd nd nd 8.932 0.082 13.26747962 403 4 15 0.02 0.005 0.175 0.18 9 9067500 136012500 8.93 15227552.6 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Of-iso-t3-8 Orbicella faveolata T3 Enriched nd nd nd 3.0 - 3.2 nd 5.279 nd nd nd 5.279 0.0676 10.93758076 251 4 18 0.02 0.005 0.175 0.18 9 5647500 101655000 5.28 19256488 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Of-iso-t6-1 Orbicella faveolata T6 Control nd nd nd 2.3 - 2.8 nd 9.85 nd nd nd 9.85 0.0443 7.167674964 248 4 28 0.02 0.005 0.175 0.18 9 5580000 156240000 9.85 15861928.9 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Picture taken of sample. Of-iso-t6-2 Orbicella faveolata T6 Enriched nd nd nd 5.1 - 6.2 nd 13.517 nd nd nd 13.517 0.1581 25.5803479 929 4 25 0.02 0.005 0.175 0.18 9 20902500 522562500 13.52 38659650.8 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Of-iso-t6-3 Orbicella faveolata T6 Control nd nd nd 3.6 - 3.8 nd 11.526 nd nd nd 11.526 0.0751 12.15106975 418 4 25 0.02 0.005 0.175 0.18 9 9405000 235125000 11.53 20399531.5 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Of-iso-t6-4 Orbicella faveolata T6 Control nd nd nd 3.0 - 3.7 nd 5.634 nd nd nd 5.634 0.0702 11.35825694 419 4 28 0.02 0.005 0.175 0.18 9 9427500 263970000 5.63 46853035.1 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Picture taken of sample. Of-iso-t6-5 Orbicella faveolata T6 Enriched nd nd nd 3.2 - 3.4 nd 12.218 nd nd nd 12.218 0.0678 10.96994046 180 4 20 0.02 0.005 0.175 0.18 9 4050000 81000000 12.22 6629562.9 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Picture taken of sample. Of-iso-t6-6 Orbicella faveolata T6 Enriched nd nd nd 4.3 - 4.5 nd 12.056 nd nd nd 12.056 0.1099 17.78165866 425 4 22 0.02 0.005 0.175 0.18 9 9562500 210375000 12.06 17449817.5 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Of-iso-t6-7 Orbicella faveolata T6 Control nd nd nd 2.8 - 3.4 nd 9.671 nd nd nd 9.671 0.0552 8.931278962 300 4 33 0.02 0.005 0.175 0.18 9 6750000 222750000 9.67 23032778.4 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water. Of-iso-t6-8 Orbicella faveolata T6 Enriched nd nd nd 3.3 - 3.7 nd 14.502 nd nd nd 14.502 0.0698 11.29353753 460 4 20 0.02 0.005 0.175 0.18 9 10350000 207000000 14.5 14273893.3 Sample was diluted 1:10 ratio. 20uL of sample; 5uL of trypan blue; and 175uL of DI water.